President Donald Trump asked FBI chief James Comey to end an inquiry into links between his ex-national security adviser and Russia, US media report.
"I hope you can let this go," Mr Trump reportedly told Mr Comey after a White House meeting in February, according to a memo written by the ex-FBI director.
The memo was written immediately after the meeting, a day after Michael Flynn resigned, according to US media.
The White House has denied the report in a statement.
"The president has never asked Mr Comey or anyone else to end any investigation, including any investigation involving General Flynn," it said.
Mr Flynn was forced out in February after he misled the vice-president about his conversations with Russia's ambassador before Mr Trump took office.
The latest Russian twist, first reported by the New York Times, comes a week after Mr Trump fired Mr Comey over his handling of the FBI investigation into Hillary Clinton's use of a private email server while at the state department.
Mr Comey's dismissal sent shockwaves through Washington, with critics accusing the president of trying to thwart the FBI investigation into Russia's alleged interference in the US election and any Moscow ties to Trump associates.